The early detection of the Citrus tristeza virus (CTV) in Citrus is crucial and is conducted using numerous techniques. Its elimination is reached through thermotherapy combined with shoot-tip grafting which is presently considered the most effective. The current study is enshrined in this context and aims to (i) optimize the STG technique for the elimination of CTV on infected Satsuma Mandarins cultivar Okitsu, (ii) evaluate the health status of STG treated plants by comparing real-time LAMP, DTBIA, ELISA, and biological indexing and finally (iii) develop a system for CTV detection based on the combination of plant tissue-print on nitrocellulose membrane coupled to real-time LAMP. The used STG protocol yielded a low plant survival rate of 5% in Satsuma Mandarins cv. Okitsu. It required 70 days instead of 182 days as reported in literature. Real-time LAMP was rated most accurate and rapid since the time dedicated was 2 hours instead of 30, 34, 1460 hours for DTBIA, ELISA and Biological indexing, respectively. CTV detection tests using membranes indicated that 20 µl of extraction buffer with 10 minutes of incubation period gave the best results. This study, being the first report on the application of real-time LAMP using plant prints on nitrocellulose membrane, shows that the test is promising and that further research should be conducted to optimize the protocol.